血清蛋白に關する「ポーラログラフ」的硏究(第2報) : アルカリ変性について(続)

Abstract

1. In the preceding paper we showed that the course of denaturation with serum protein implies two different successive processes (i. e. activation and inactivation), from the view point of polarographic protein study. Now we succeeded to separate this two phases of denaturation into two independent reactions under a suitable temperature; namely below 20℃ the protein wave keeps almost constant wave span, while above this temparature it decreases rapidly. Considering that the aim of Brdicka test is to find the total active SH of serum protein, it must be carried out at this lower temperature. At higher temperature, it will be a new test for degradation of SH activity which has its certain clinical significance. 2. Owing to the blood proteose another new protein double wave can be obtained in the sulfosalicylic acid filtrate. Examing the alkaline denaturation of this filtrate in regard to time, we found in the majority of case (61 cases out of 66 cases of different sera) that the wave height was once minimized in 30'--60' after KOH addition (0.08N) and then raised, showing, therefore, an inverse relation to the case of serum protein. But in the remaining 5 cases, in which 3 was with nephritis, the proteose showed similar denaturation to that of serum protein. The reliability of these phenomenona will be definite, because the results of repeated observation over 5 months of periods fell within a narrow range of values. From this fact, it may be said that the blood proteose of some persons, especially nephrotic, has a characteristic closely akin to the serum protein.