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タイトル: Basic fibroblast growth factor promotes the generation of microtubule-associated protein 2-positive cells from microglia
著者: Niidome, Tetsuhiro
Nonaka, Hideki
Akaike, Akinori
Kihara, Takeshi
Sugimoto, Hachiro
著者名の別形: 新留, 徹広
キーワード: Basic fibroblast growth factor
Differentiation
Fibroblast growth factor receptor
Glial fibrillary acidic protein
Microglia
Microtubule-associated protein 2
発行日: 18-Dec-2009
出版者: Elsevier
誌名: Biochemical and Biophysical Research Communications
巻: 390
号: 3
開始ページ: 1018
終了ページ: 1022
抄録: We recently demonstrated that microglia as multipotential stem cells give rise to microtubule-associated protein 2 (MAP2)-positive and glial fibrillary acidic protein (GFAP)-positive cells and that microglia-derived MAP2-positive cells possess properties of functional neurons. In this study, we investigated the role of fibroblast growth factor (FGF) signaling in the molecular mechanism underlying the generation of microglia-derived MAP2-positive and GFAP-positive cells. Real-time quantitative PCR analyses demonstrated that mRNA levels of a family of three FGF receptors, Fgfr1-3, were upregulated in microglia treated with 70% fetal bovine serum (FBS). Immunocytochemical analyses demonstrated that basic FGF (bFGF) promoted the generation of microglia-derived MAP2-positive and GFAP-positive cells, and the FGF receptor tyrosine kinase inhibitor SU5402 and the MEK inhibitor PD98059 both inhibited this process. Western blot analyses demonstrated that bFGF increased phosphorylated ERK1/2 levels without altering total ERK1/2 levels. These results suggest that bFGF promotes the generation of microglia-derived MAP2-positive and GFAP-positive cells via FGF receptors and the ERK-MAP kinase pathway.
著作権等: c 2009 Elsevier Inc. All rights reserved.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/88960
DOI(出版社版): 10.1016/j.bbrc.2009.10.100
出現コレクション:学術雑誌掲載論文等

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