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タイトル: | Basic fibroblast growth factor promotes the generation of microtubule-associated protein 2-positive cells from microglia |
著者: | Niidome, Tetsuhiro Nonaka, Hideki Akaike, Akinori Kihara, Takeshi Sugimoto, Hachiro |
著者名の別形: | 新留, 徹広 |
キーワード: | Basic fibroblast growth factor Differentiation Fibroblast growth factor receptor Glial fibrillary acidic protein Microglia Microtubule-associated protein 2 |
発行日: | 18-Dec-2009 |
出版者: | Elsevier BV |
誌名: | Biochemical and Biophysical Research Communications |
巻: | 390 |
号: | 3 |
開始ページ: | 1018 |
終了ページ: | 1022 |
抄録: | We recently demonstrated that microglia as multipotential stem cells give rise to microtubule-associated protein 2 (MAP2)-positive and glial fibrillary acidic protein (GFAP)-positive cells and that microglia-derived MAP2-positive cells possess properties of functional neurons. In this study, we investigated the role of fibroblast growth factor (FGF) signaling in the molecular mechanism underlying the generation of microglia-derived MAP2-positive and GFAP-positive cells. Real-time quantitative PCR analyses demonstrated that mRNA levels of a family of three FGF receptors, Fgfr1-3, were upregulated in microglia treated with 70% fetal bovine serum (FBS). Immunocytochemical analyses demonstrated that basic FGF (bFGF) promoted the generation of microglia-derived MAP2-positive and GFAP-positive cells, and the FGF receptor tyrosine kinase inhibitor SU5402 and the MEK inhibitor PD98059 both inhibited this process. Western blot analyses demonstrated that bFGF increased phosphorylated ERK1/2 levels without altering total ERK1/2 levels. These results suggest that bFGF promotes the generation of microglia-derived MAP2-positive and GFAP-positive cells via FGF receptors and the ERK-MAP kinase pathway. |
著作権等: | c 2009 Elsevier Inc. All rights reserved. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/88960 |
DOI(出版社版): | 10.1016/j.bbrc.2009.10.100 |
PubMed ID: | 19854155 |
出現コレクション: | 学術雑誌掲載論文等 |
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