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Title: A novel strictly NADPH-dependent Pichia stipitis xylose reductase constructed by site-directed mutagenesis.
Authors: Khattab, Sadat Mohammad Rezq
Watanabe, Seiya
Saimura, Masayuki
Kodaki, Tsutomu  kyouindb  KAKEN_id
Author's alias: 小瀧, 努
Keywords: Coenzyme specificity
Xylose reductase
Site-directed mutagenesis
Issue Date: 14-Jan-2011
Publisher: Elsevier Inc.
Journal title: Biochemical and biophysical research communications
Volume: 404
Issue: 2
Start page: 634
End page: 637
Abstract: Xylose reductase (XR) and xylitol dehydrogenase (XDH) are the key enzymes for xylose fermentation and have been widely used for construction of a recombinant xylose fermenting yeast. The effective recycling of cofactors between XR and XDH has been thought to be important to achieve effective xylose fermentation. Efforts to alter the coenzyme specificity of XR and HDX by site-directed mutagenesis have been widely made for improvement of efficiency of xylose fermentation. We previously succeeded by protein engineering to improve ethanol production by reversing XDH dependency from NAD(+) to NADP(+). In this study, we applied protein engineering to construct a novel strictly NADPH-dependent XR from Pichia stipitis by site-directed mutagenesis, in order to recycle NADPH between XR and XDH effectively. One double mutant, E223A/S271A showing strict NADPH dependency with 106% activity of wild-type was generated. A second double mutant, E223D/S271A, showed a 1.27-fold increased activity compared to the wild-type XR with NADPH and almost negligible activity with NADH.
Rights: © 2010 Elsevier Inc.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
DOI(Published Version): 10.1016/j.bbrc.2010.12.028
PubMed ID: 21146502
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