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タイトル: | Facile conversion of RNA aptamers to modular fluorescent sensors with tunable detection wavelengths. |
著者: | Nakano, Shun Nakata, Eiji https://orcid.org/0000-0001-9564-6805 (unconfirmed) Morii, Takashi https://orcid.org/0000-0003-3663-3267 (unconfirmed) |
著者名の別形: | 森井, 孝 |
キーワード: | Aptamer Ribonucleopeptide Biosensor Modular design Fluorescence detection |
発行日: | 1-Aug-2011 |
出版者: | Elsevier Ltd. |
誌名: | Bioorganic & medicinal chemistry letters |
巻: | 21 |
号: | 15 |
開始ページ: | 4503 |
終了ページ: | 4506 |
抄録: | A GTP aptamer was converted to a modular fluorescent GTP sensor by conjugation of RRE (Rev responsive element) RNA and successive complex formation with a fluorophore-modified Rev peptide. Structural changes associated with substrate binding in the RNA aptamer were successfully transduced into changes in fluorescence intensity because of the modular structure of ribonucleopeptides. A simple modular strategy involving conjugation of a fluorophore-modified ribonucleopeptide to the stem region of an RNA aptamer deduced from secondary structural information helps produce fluorescent sensors, which allow tuning of excitation and detection wavelengths through the replacement of the fluorophore at the N-terminal of the Rev peptide. |
著作権等: | © 2011 Elsevier Ltd. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/147154 |
DOI(出版社版): | 10.1016/j.bmcl.2011.05.120 |
PubMed ID: | 21719284 |
出現コレクション: | 学術雑誌掲載論文等 |
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