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j.celrep.2018.06.013.pdf | 6.2 MB | Adobe PDF | 見る/開く |
タイトル: | MTSS1 Regulation of Actin-Nucleating Formin DAAM1 in Dendritic Filopodia Determines Final Dendritic Configuration of Purkinje Cells |
著者: | Kawabata Galbraith, Kelly Fujishima, Kazuto Mizuno, Hiroaki Lee, Sung-Jin Uemura, Takeshi Sakimura, Kenji Mishina, Masayoshi Watanabe, Naoki https://orcid.org/0000-0001-8492-200X (unconfirmed) Kengaku, Mineko https://orcid.org/0000-0002-1666-3648 (unconfirmed) |
著者名の別形: | 川端 Galbraith, ケリー 藤島, 和人 水野, 裕昭 李, 聖真 植村, 健 崎村, 建司 三品, 昌美 渡邊, 直樹 見学, 美根子 |
キーワード: | dendrite actin Purkinje cell neuron filopodia spine ARP2/3 formin MTSS1 DAAM1 |
発行日: | 3-Jul-2018 |
出版者: | Elsevier BV |
誌名: | Cell Reports |
巻: | 24 |
号: | 1 |
開始ページ: | 95 |
終了ページ: | 106.e9 |
抄録: | Dendritic filopodia of developing neurons function as environmental sensors, regulating the spatial organization of dendrites and proper targeting to presynaptic partners. Dendritic filopodia morphology is determined by the balance of F-actin assembled via two major nucleating pathways, the ARP2/3 complex and formins. The inverse-BAR protein MTSS1 is highly expressed in Purkinje cells (PCs) and has been shown to upregulate ARP2/3 activity. PCs in MTSS1 conditional knockout mice showed dendrite hypoplasia due to excessive contact-induced retraction during development. This phenotype was concomitant with elongated dendritic filopodia and was phenocopied by overactivation of the actin nucleator formin DAAM1 localized in the tips of PC dendritic protrusions. Cell biology assays including single-molecule speckle microscopy demonstrated that MTSS1’s C terminus binds to DAAM1 and paused DAAM1-mediated F-actin polymerization. Thus, MTSS1 plays a dual role as a formin inhibitor and ARP2/3 activator in dendritic filopodia, determining final neuronal morphology. |
記述: | アクチン繊維のわずかな偏りが脳神経細胞の形を決めることを発見しました. 京都大学プレスリリース. 2018-07-17. |
著作権等: | © 2018 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
URI: | http://hdl.handle.net/2433/232641 |
DOI(出版社版): | 10.1016/j.celrep.2018.06.013 |
PubMed ID: | 29972794 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2018-07-17 |
出現コレクション: | 学術雑誌掲載論文等 |
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