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Title: MTSS1 Regulation of Actin-Nucleating Formin DAAM1 in Dendritic Filopodia Determines Final Dendritic Configuration of Purkinje Cells
Authors: Kawabata Galbraith, Kelly
Fujishima, Kazuto  KAKEN_id
Mizuno, Hiroaki
Lee, Sung-Jin
Uemura, Takeshi
Sakimura, Kenji
Mishina, Masayoshi
Watanabe, Naoki  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-8492-200X (unconfirmed)
Kengaku, Mineko  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-1666-3648 (unconfirmed)
Author's alias: 川端 Galbraith, ケリー
藤島, 和人
水野, 裕昭
李, 聖真
植村, 健
崎村, 建司
三品, 昌美
渡邊, 直樹
見学, 美根子
Keywords: dendrite
actin
Purkinje cell
neuron
filopodia
spine
ARP2/3
formin
MTSS1
DAAM1
Issue Date: 3-Jul-2018
Publisher: Elsevier BV
Journal title: Cell Reports
Volume: 24
Issue: 1
Start page: 95
End page: 106.e9
Abstract: Dendritic filopodia of developing neurons function as environmental sensors, regulating the spatial organization of dendrites and proper targeting to presynaptic partners. Dendritic filopodia morphology is determined by the balance of F-actin assembled via two major nucleating pathways, the ARP2/3 complex and formins. The inverse-BAR protein MTSS1 is highly expressed in Purkinje cells (PCs) and has been shown to upregulate ARP2/3 activity. PCs in MTSS1 conditional knockout mice showed dendrite hypoplasia due to excessive contact-induced retraction during development. This phenotype was concomitant with elongated dendritic filopodia and was phenocopied by overactivation of the actin nucleator formin DAAM1 localized in the tips of PC dendritic protrusions. Cell biology assays including single-molecule speckle microscopy demonstrated that MTSS1’s C terminus binds to DAAM1 and paused DAAM1-mediated F-actin polymerization. Thus, MTSS1 plays a dual role as a formin inhibitor and ARP2/3 activator in dendritic filopodia, determining final neuronal morphology.
Description: アクチン繊維のわずかな偏りが脳神経細胞の形を決めることを発見しました. 京都大学プレスリリース. 2018-07-17.
Rights: © 2018 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
URI: http://hdl.handle.net/2433/232641
DOI(Published Version): 10.1016/j.celrep.2018.06.013
PubMed ID: 29972794
Related Link: https://www.kyoto-u.ac.jp/ja/research-news/2018-07-17
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