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タイトル: Plastic roles of phenylalanine and tyrosine residues of an RGG motif of TLS/FUS in binary and ternary complex formation with the G-quadruplex structures of telomeric DNA and TERRA
著者: Kondo, Keiko
Mashima, Tsukasa  kyouindb  KAKEN_id
Oyoshi, Takanori
Yagi, Ryota
Kurokawa, Riki
Kobayashi, Naohiro
Nagata, Takashi  kyouindb  KAKEN_id
Katahira, Masato  kyouindb  KAKEN_id
著者名の別形: 近藤, 敬子
真嶋, 司
永田, 崇
片平, 正人
発行日: 12-Feb-2018
出版者: Springer Nature
誌名: Scientific reports
巻: 8
論文番号: 2864
抄録: The length of a telomere is regulated via elongation and shortening processes. Telomeric DNA and telomeric repeat-containing RNA (TERRA), which both contain G-rich repeated sequences, form G-quadruplex structures. Previously, translocated in liposarcoma (TLS) protein, also known as fused in sarcoma (FUS) protein, was found to form a ternary complex with the G-quadruplex structures of telomeric DNA and TERRA. We then showed that the third RGG motif of TLS, the RGG3 domain, is responsible for the complex formation. However, the structural basis for their binding remains obscure. Here, NMR-based binding assaying revealed the interactions in the binary and ternary complexes of RGG3 with telomeric DNA or/and TERRA. In the ternary complex, tyrosine bound exclusively to TERRA, while phenylalanine bound exclusively to telomeric DNA. Thus, tyrosine and phenylalanine each play a central role in the recognition of TERRA and telomeric DNA, respectively. Surprisingly in the binary complexes, RGG3 used both tyrosine and phenylalanine residues to bind to either TERRA or telomeric DNA. We propose that the plastic roles of tyrosine and phenylalanine are important for RGG3 to efficiently form the ternary complex, and thereby regulate the telomere shortening.
著作権等: © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
URI: http://hdl.handle.net/2433/234273
DOI(出版社版): 10.1038/s41598-018-21142-1
PubMed ID: 29434328
出現コレクション:学術雑誌掲載論文等

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